Products/Rapid-Tests veterinary/Foal IgG

Vet-o-test

Foal IgG

VET521

Rapid test for whole blood, serum or plasma specimens

Introduction 
The objective of the Vet-o-test Foal IgG test kit is to determine Foal IgG antibodies titer in foal. Antibody titer against Foal IgG is measured by solid phase immunochromatographic assay for the rapid and semi-quantitative detection immunoglobulin G (IgG) in foal whole blood, serum or plasma. Protection in newborn foal is mainly by maternally derived antibodies. The maternally derived antibodies strongly protect foal from infection. Thus, levels of maternal derived antibodies can help diagnose what extent.
 
Principles
Vet-o-test Foal IgG test kit is designed to detect the IgG of foal in whole blood, serum or plasma. After being absorbed into the cellulose pad, the IgG of foal move and bind with goldcolloid complex of goat anti-horse IgG of the conjugate pad, forming Ab-Ab complex. This complex, then, forms Ab-Ab-Ab direct sandwich binding with the antibodies of foal IgG in the nitrocellulose membrane. The test results can be shown through the appearance of Control and Test lines where the principles of immunochromatography are used.
 
Characteristics

  1. One-step rapid test of Foal IgG antibodies
  2. Rapid test results between 5 ~ 10 minutes
  3. Expensive equipment not required
  4. Easy storage and maintenance
  5. High-purity and high-quality materials of the test kit increase its sensitivity and specificity.

 
Materials (1 test/kit)

  1. Test --------------------------------------------------------------------------------------- 1 unit
  2. Diluent (buffer) --------------------------------------------------------------------------16 ㎖ x 1 unit 
  3. Loading buffer ---------------------------------------------------------------------------2 ㎖ x 1 unit
  4. Disposable loops --------------------------------------------------------------------- 1 units

 
Composition
Specimen well (S : for dropping), Test line (T), and Control line (C) are marked on the device. Inside of it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test paper), and absorbent pad. 
 
Effect
Detection of newborn foal IgG from foal whole blood, serum or plasma 
 
Uses

  1. Specimen
    Foal whole blood serum or plasma
  2. Test procedure
    a)  When specimen and test kits are stored at cold circumstances (2~8 ℃), put them at room temperature for 15~30 minutes before use.
    b)  Take out a device from a pouch and place it on a horizontal surface.
    c)  Using a loop, carefully immerse only the loop tip into the sample . Visually confirm that the loop is filled
    d)  Transfer the filled loop by immersing and twirling the loop tip in the bottle of sample diluent.
    e)  Mix enough sample and diluent buffer by inverting the tube. Hold the sample diluent bottle vertically and dispose of the first 3~5 drops from the bottle.
    f)  Using a bottle , dispense 1drop of the fluid into the specimen well (S), add 1 drops  ( 40 ㎕ ) of loading buffer.
    g)  Read the test results between 5 ~ 10 minutes.
  3.  Interpretation of the results
    A purple band should appear on the control line regardless of the test result. The presence of another band on the test line determines the result.
    Control Line (C) : The line should always appear regardless of the presence of foal IgG.
    If this line does not appear, the test should be considered invalid. This might be because of deterioration of the kit or the lack of specimen. It should be tested again with another kit.
    Test Line (T) :   The titer of foal IgG antibodies is determined by  Comparing the intensities of lines between T line and C line.
    a)  Less than 400mg/dL IgG ( < 400mg/dL )
    Auswertung des Fohlen IgG Schnelltests
    b)  400 to 800mg/dL ( 400-800 mg/dL )
    Auswertung des Fohlen IgG Schnelltests
    c)  Approximately 800mg/dL IgG
    Auswertung des Fohlen IgG Schnelltests
    d)  Greater than 800mg/dL IgG ( > 800mg/dL )
    Auswertung des Fohlen IgG Schnelltests
    e)  Retesting
    i) Control lines do not appear.             ii) Test line only appears.
    Auswertung des Fohlen IgG Schnelltests
  4.  Example of Test Results
    Line Intensity ( C vs. T )IgG LevelInterpretation
    Auswertung des Fohlen IgG Schnelltests C>>T< IgG 400Supplement with colostrum
     Auswertung des Fohlen IgG SchnelltestsC > TIgG400-800mg/dLMonitor foal.
    Supplement if foal shows signs of disease, or weakness.
     Auswertung des Fohlen IgG SchnelltestsC = T  IgG = 800 mg/dLNormal
     Auswertung des Fohlen IgG SchnelltestsC < TIgG > 800Very good transfer

Further examinations
This test is for primary screening only. Consult veterinarians for further necessary examinations to obtain clinical test results.
 
Precautions

  1. Use for foal in-vitro diagnostic purposes only. 
  2. Use within 10 minutes after opening the pouch because the test kit is very sensitive to  moisture and its effect may diminish.    
  3. Be careful of not touching the result window.
  4. Every specimen should be used with different droppers.
  5. For testing, the buffer included should be used.
  6. Do not use specimen showing hemolysis or being contaminated with microbes, which may  cause invalid results.
  7. Deal with specimen carefully. They can deliver unknown virus or infectious bacteria.
  8. Use disposable gloves when you suspect the infection caused by specimen. And wash your   hands later.  
  9. Dispose solid wastes after sterilizing them at 121 ℃ for over 1 hour.
  10. Do not use the kit when its pouch is torn, sealing is not good in shape or expiration date is passed.


Storage method 
The test kits stored at 2~30℃ can be used for 24 months after manufacturing. Do not keep them in a refrigerator. However, if they are stored under cold circumstances, keep them at room temperature for 15 ~ 30 minutes before use.
 
Exchange
The test kits are manufactured under strict quality control system. Nevertheless, if they are deteriorated during delivery, ask our distributors for exchange.
 
Liability
The entire risk due to the performance of this product is assumed by the purchaser. The manufacturer shall not be liable for indirect, special or consequential damages of any kind resulting from use of this product.
 

Instruction for Foal IgG test

Impfschleife
Loop tip: Immerse the loop tip only.
 
 1
Sampling

Probenverarbeitung des Fohlen IgG-Schnelltests
Transfer the filled loop by immersing and twiring the loop tip in the bottle od the sample diluent
 
 2
Mixing

 
Mischen der Probe

Mix enough sample and diluent buffer by inverting the tube
Verwerfen der ersten Tropfen

Hold the sample diluent bottle vertically and dispose of the first 3-5 drops from bottle
 
3
Loading

Eintropfen der Probe
1 drop of buffer
**after complete absorption

 Zugabe des Reaktionspuffers
1 drop of Loading buffer

 
4
Reading
 
Wartezeit 5 bis 10 Minuten
Positives Testergebnis
Read the result between 5-7 min
Negatives Testergebnis
 
5
Discard

 
nach mehr als 10 Minuten test nicht mehr auswerten
Discard the used device
**invalid result after 10 min
Beachten Sie die Auswertezeit

 

img/page/needfull/ce-zeichen.gif Manufacturer:

Guenter Keul GmbH
Günter Keul GmbH
Von-Langen-Weg 10
D-48565 Steinfurt
Tel.: 02551/2097  Fax.: 02551/80883
Web: www.keul.de

 



 
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