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Produkte/Mikrobiologie - LabM
Chromogenic Culture Media
 | Harlequin™ Salmonella ABC (Freeman Formulation) - HAL001
Harlequin™ Salmonella ABC was developed for the isolation of Salmonella spp. from food and clinical samples. The medium utilises a dual chromogen system to differentiate between Salmonella and other members of the family Enterobacteriaceae. Most traditional Salmonella isolation media are highly non-specific and consequently place a heavy burden on the laboratory in terms of the biochemical and serological confirmation of suspect colonies. With improved specificity, ABC medium dramatically reduces the need for ’false-positive’ screening, saving labour and reducing consumable costs.
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Harlequin™ C.L.E.D. Medium - HAL007
A chromogenic version of traditional C.L.E.D., HAL007 allows easier identification of organisms known to be the cause of urinary tract infections. Superior growth qualities and the isolation of more clinical pathogens than traditional C.L.E.D., make HarlequinTM C.L.E.D. the first choice for screening. |
Harlequin™ TBGA (Tryptone Bile Glucuronide Agar) - HAL003
A medium developed for the simple enumeration of E. coli without the need for membranes, or pre-incubation on Minerals Modified Glutamate Medium. Based upon the formulation of Tryptone Bile Agar, LAB072, the medium has been modified by the addition of a chromogenic substrate to detect the ß-glucuronidase enzyme, which is highly specific for E. coli, facilitating easier enumeration in the presence of other organisms, or when large numbers are present on the plate. |  |
Harlequin™ mLGA (membrane Lactose Glucuronide Agar) - HAL009
Traditionally, membrane Lauryl Sulphate Broth (mLSB) has been used as the standard media for isolating coliforms (including E. coli) from water potentially contaminated with sewage. Harlequin™ membrane Lactose Glucuronide Agar (mLGA) is a modification of mLSB (LAB082) aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and E. coli. The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide, sodium pyruvate and agar. The incorporation of X-glucuronide allows for the presumptive isolation of E. coli, sodium pyruvate aids the recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads. This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Microbiology of Drinking Water 2002. |  |
| Harlequin™ E. coli/Coliform Medium - HAL008
This dual chromogenic substrate medium has been developed for the simultaneous enumeration of Escherichia coli and coliforms in food and environmental samples. The different colony types are simple to distinguish allowing rapid counting of both E. coli and coliforms on a single medium. Based upon the formulation of Tryptone Bile Agar, LAB072, the medium has been modified by the addition of two chromogenic substrates, one to detect the β-glucuronidase enzyme (X-glucuronide) and another to detect the β-galactosidase enzyme (magenta-β-gal). |
Harlequin™ SMAC-BCIG - HAL006
Designed to reduce ‘false-positive’ results when isolating E.coli O157 amongst other, more commonly occuring, non-sorbitol fermenting E.coli. E.coli O157 remains colourless, whilst other organisms produce colours from pink, to pink with a purple centre, or purple, depending upon their ability to utilise sorbitol and β-glucuronide. |  |
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Harlequin™ Listeria Chromogenic Agar - HAL010
As detailed in ISO 11290, Harlequin Listeria Chromogenic Agar (Agosti and Ottaviani formulation) when prepared with the required selective and differential supplements is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials. The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of a phospholipase enzyme that will be present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies. With the combination of both a chromogenic and a phospholipase enzyme reaction, it is possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo). |
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Aeromonas Agar
Aeromonas Agar Bile Salt Irgasan Brilliant Green Agar LAB 167 Interpretation Organism Size Shape | Colour | Aeromonas spp.* 0.5-3.0 CV.E.G | Translucent pink | Pseudomonas spp. 0.5-1.0 CV.E |
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Anaerobe Identification Base
Anaerobe Identification Medium Base LAB 66 Description A medium introduced by Phillips in 1976 for testing the fermentation capabilities of non-sporing anaerobes. The base is carbohydrate free and nutritious.
Method for reconstitution Weigh 17.5 grams of powder and disperse in 1 ... |
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Aseptic Commissioning Broth
Aseptic Commissioning Broth LAB 157 Description A general growth medium specifically designed for commissioning of aseptic filling machines in the pharmaceutical allied industries. It is a simple formulation incorporating pepto yeast extract and sucrose as energy sources ... |
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Bacillus Cereus Medium
Bacillus Cereus Medium Phenol Red Egg Yolk Polymyxin Agar ( P.R.E.P. ) Mannitol Egg Yolk Polymyxin Agar LAB 73 Description Introduced by Mossel and his co-workers in 1967 for the enumeration of Bacillus cereus in foods, this formula was shown to be the most effective for ... |
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Baird-Parker Medium Base
Baird-Parker Medium Base LAB 85 Description Baird-Parker introduced this complex medium in 1962 to overcome the problems of recovering damaged Staphylococcus aureus from foodstuffs. The medium is highly selective due to potassium tellurite and lithium chloride. The tellurite ... |
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Blood Free Selective Medium
Campylobacter Blood Free Selective Medium (Modified CCDA-Improved) LAB 112 Description A blood free medium which will support the growth of most enteric campylobacters. The selective cocktail X112 (X212) makes the medium selective for C. jejuni. C. coli and C. laridis ... |
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Brain Heart Infusion Agar
Description A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. The mixture of brain and heart infusions is particularly useful in the isolation of Actinomyces israeli and Histoplasma capsulatum. With the addition of 7% defibrinated ... |
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Brazier’s CCEY Agar
Brazier's CCEY Agar LAB 160 Description Brazier's CCEY agar is the formulation currently used by the Anaerobe Reference Unit for the isolation of C.difficile, resulting from work initiated by Ken Phillips and Paul Levett, and completed by Jon Brazier. Based upon the market ... |
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Brilliant Green Agar (modified)
Brilliant Green Agar (modified) (Phenol Red Brilliant Green Agar, BPLS) LAB 34 Description First introduced by Kristensen et al in 1925 as a selective medium for the isolation of salmonellae (except S. typhi). The medium was modified by the Netherlands Institute for Public ... |
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Bromocresol Purple Lactose Agar
Bromocresol Purple Lactose Agar (Drigalski agar)
Description A modification of MacConkey's medium, formulated in 1926 by Dunham and Schoenlein, for the recovery of coliform bacteria in foodstuffs and water. The brilliant green and bile inhibit most Gram positive organisms ... |
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Buffered Listeria Enrichment Broth
Buffered Listeria Enrichment Broth LAB 139 Description A medium for the selective enrichment of food and environmental samples for Listeria spp, LAB 139 is a buffered version of the ‘FDA' broth LAB 138. The extra buffering capacity maintains the pH of the enrichment culture ... |
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Buffered Peptone Water
Buffered Peptone Water LAB 46 Description A pre-enrichment medium design to help sublethally damaged salmonellae recover before introducing them into a selective medium. This nutrient medium is free from inhibitors and is well buffered to maintain the pH at 7.2 for the incubation ... |
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C.E.M.O. Agar Base
C.E.M.O. Agar Base (Contagious Equine Metritis Organism) LAB 78 Description This medium is a selective isolation medium for Taylorella equigenitalis the causative organism of contagious equine metritis. The medium is a sugar free base with a mixture of high grade casein and ... |
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C.L.E.D. (Bevis modification)
C.L.E.D. (Bevis modification) (Cystine Lactose Electrolyte Deficient - Double Indicator) LAB 6 Description Bevis modified Mackey and Sandys original medium by introducing a double indicator to improve the differentiation of lactose and non lactose fermenting coliforms ... |
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C.L.E.D. Medium
C.L.E.D. Medium Formula | g/litre | Balanced Peptone No. 1 | 4.0 | Beef Extract | 3 |
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Campylobacter Enrichment Broth
Campylobacter Enrichment Broth (Bolton Formulation) LAB 135 Description A selective enrichment broth for the isolation of Campylobacter spp. from food, environmental samples and faeces. The use of a selective enrichment broth enhances the recovery of sub-lethally damaged ... |
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Cetrimide Agar
Cetrimide Agar U.S.P. LAB 133 Description A medium recommended by the United States Pharmacopoeia for the isolation of Pseudomonas aeruginosa from pharmacological preparations. Subculture is carried out onto the medium after enrichment in LAB 4 Tryptone Soy Broth. Cetrimide ... |
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China Blue Lactose Agar
China Blue Lactose Agar LAB 105 Description China Blue Lactose Agar is a non-inhibitory medium for the differentiation and enumeration of bacteria in milk. The formulation is the same as that described by the Methodenkomission für Milchivirtschaft. Lactose fermenters in milk form ... |
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Chloramphenicol Agar Base
Rose Bengal Chloramphenicol Agar Base LAB 36 Description A selective medium for the enumeration of moulds and yeasts in foods. The original formulation of Jarvis (1973) used chlortetracycline, this has been substituted by chloramphenicol because of superior selectivity. The Rose ... |
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Columbia Agar Base
Columbia Agar Base LAB 1 Description A general purpose nutritious agar base formulated by Ellner et al. When further enriched by the addition of sterile blood, Columbia agar can be used for the isolation of most clinically significant pathogens. The blood can be &lsquo ... |
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