LAB 66

Description

A medium introduced by Phillips in 1976 for testing the fermentation capabilities of non-sporing anaerobes. The base is carbohydrate free and nutritious.

Method for reconstitution

Weigh 17.5 grams of powder and disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix and heat gently to dissolve. Sterilise at 121˚C for 15 minutes, or alternatively, by filtration.

Appearance: Red, clear liquid.

pH: 7.2 + 0.2

Method for reconstitution

Weigh 40 grams of powder, disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix then sterilise by autoclaving at 121˚C for 15 minutes. Cool to 47˚C and aseptically add 50-70ml of sterile defibrinated horse blood. Mix well and pour. Before use, flood the surface with 1ml of a sterile solution of the substrate under test.

Appearance: Blood agar plate.

pH: 7.2 ± 0.2

Minimum Q.C. organisms: Escherichia coli (acid and gas production) NCIMB 50034 Proteus spp. (turbidity, no acid or gas)

Inoculation: Dispense medium through filling line into the final packaging, or suitable container, as appropriate.

Incubation: As per routine sterility testing laid down in Pharmacopoeias or in house methodology. Typically 20-25˚C for up to 14 days.

Minimum Q.C. organisms: B. fragilis