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Produkte/Mikrobiologie - LabM/Media Range/C.L.E.D. Medium

C.L.E.D. Medium

Formula	g/litre
Balanced Peptone No. 1	4.0
Beef Extract	3.0
Tryptone	4.0
Lactose	10.0
L-Cystine	0.128
Bromothymol blue indicator	0.02
Andrade's indicator	0.08
Agar No. 1	15.0

Method for reconstitution

Weigh 36 grams of powder, disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix then sterilise by autoclaving for 15 minutes at 121˚C. Cool to 47˚C and mix before pouring.

Appearance: Green/blue, clear gel.

pH: 7.5 ± 0.2

Minimum Q.C. organisms: E. coli

NCIMB 50034 S. aureus NCIMB 50080

Storage of Prepared Medium: Plates - up to 7 days at 2-8˚C in the dark.

Inoculation method: Surface inoculation, either streaking for single colonies or spread evenly over entire surface for colony counts.

Incubation: 37˚C for 24 hours aerobically.

Growth Characteristics colony size shape & organism (mm) surface colour			other
E. coli 2.0-3.0	CV.E.G.	Yellow/ Orange	(Blue if non-lactose fermenter)
K. aerogenes 3.0-4.0	CV.E.G.	Yellow/ Orange	mucoid
Proteus spp. 2.0-3.0	CV.E.G.	Blue
Ps. aeruginosa 1.0-4.0	F.CR.D.	Blue	(Green pigment & odour)
Shigella spp. 1.5-2.5	CV.E.G.	Blue
Salmonella spp. 2.0-3.0	CV.E.G.	Blue	(Yellow-orange if lactose +ve)
S. aureus 1.0-1.5	CV.E.G.	Yellow/ Orange	(Blue if non-lactose fermenting)
Other staphylococci 0.5-1.5	CV.E.G.	Blue-White	(Yellow if lactose fermenting)
Enterococcus spp. 0.5	CV.E.G.	Yellow-Orange

(Mackey and Sandys)

(Cystine Lactose Electrolyte Deficient-Single Indicator)

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DIN EN ISO 13485:2003
EN ISO 13485:2003