China Blue Lactose Agar LAB 105 Description China Blue Lactose Agar is a non-inhibitory medium for the differentiation and enumeration of bacteria in milk. The formulation is the same as that described by the Methodenkomission für Milchivirtschaft. Lactose fermenters in milk form blue colonies. The medium is non-inhibitory to the growth of cocci, making it a useful medium for the detection of streptococci and staphylococci as well as coliform organisms.
Method for reconstitution Weigh 45.3 grams of powder, disperse in 1 litre of deionised water. Add 10ml of glycerol, allow to soak for 10 minutes then swirl to mix. Sterilise at 121˚C for 10 minutes. Appearance: Opalescent, pale yellow agar. pH: 7.2 ± 0.2 Method for reconstitution Weigh 35 grams of powder, disperse in 1 litre of deionised water. Allow to soak for 10 minutes, swirl to mix then sterilise by autoclaving at 121˚C for 15 minutes. Cool to 47˚C and pour into Petri dishes. Appearance: Blue clear agar. pH: 7.0 ± 0.2 Minimum Q.C. organisms: E. coli NCIMB 50034 Staph. aureus NCIMB 50080 Storage of Prepared Medium: Plates - up to 7 days at 2-8˚C in the dark. Inoculation: Surface, spread inoculum evenly over entire surface. Incubation: 37˚C aerobically for 18-24 hours. Interpretation: Count all colonies
E. coli (inhibition) NCIMB 50034 Storage of Prepared Medium: Plates - up to 7 days at 2-8˚C in the dark. Inoculation: Subculture from enrichment broth, streak out for single colonies. Incubation: 30-35˚C aerobically for 24-48 hours.
References Methodenbuch Band VI. Vergand Deutscher Landwirtschaftlicher Untersuchungs - und Forschugsanstallen. |
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