MSRV

(Semi-solid Rappaport Medium)

LAB 150

Description

MSRV was developed in 1986 by De Smedt, Bolderdijk and Rapp as a rapid means of Salmonella detection. The medium, based u Rappaport Vassiliadis broth, is inoculated directly from the enrichment medium, in the centre of the plate. Motile organi spread from the centre in the semi-solid agar, but non-salmonellas inhibited by the selective agents.

After overnight incubation the use of polyvalent salmonella anti or a latex kit can confirm the presence of a Salmonella. Alternativ a paper disc wetted with polyvalent H antiserum can be placed 1/ the way from the edge of the dish, and will signal the presence Salmonella by inhibiting the mobility of the organism around disc.

Using this medium De Smedt and Bolderdijk have reported possibility of detecting Salmonella in 24hrs (1987)