Nutrient Broth "E"

LAB 68

Description

An inexpensive broth for the growth of nutritionally non-demanding organisms. Ideal for teaching purposes.

Method for reconstitution

Weigh 13 grams of powder, add to1 litre of deionised water. Heat to dissolve then dispense into bottles or tubes. Sterilise by autoclaving at 121˚C for 15 minutes.

Appearance: Straw coloured, clear.

pH: 7.4 ± 0.2

Minimum Q.C. organisms: S. aureus NCIMB 50080 E. coli NCIMB 50034

Storage of Prepared Medium: Capped containers - up to 3 months at 15-20˚C in the dark.

Inoculation and incubation: To suit chosen organism.

Growth indicator: Turbidity.