Harlequin™ LB Top Agar

HAL 5

Description

A nutritious molecular biology medium containing the novel chromogen CHE-galactoside to enable rapid, safe and unambiguous detection of phage transformed bacteria.

Formula g/litre
Tryptone 10.0
Yeast Extract 5.0
Sodium chloride 10.0
CHE-galactoside 1.0
IPTG 0.03
Ferric ammonium citrate 2.0
Agar 4.0

Method for reconstitution
Weigh 32 grams of powder and disperse in 1 litre of deionised water. Swirl to mix and sterilise by autoclaving at 121°C for 15 minutes.

Cool to 47°C before seeding the medium with required strains.

Appearance:
Straw, clear gel.

pH:
7.0 ± 0.2

Storage of Prepared Medium:
Storage of capped medium in bottles for up to 1 month at room temperature in the dark.

References
Miller, J.H. (1972). Experiments in Molecular Genetics. Cold Spring Harbour Laboratory. Cold Spring Harbour New,York. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbour Laboratory. Cold Spring Harbour New, York.