Campylobacter Enrichment Broth

(Bolton Formulation)

LAB 135

Description

A selective enrichment broth for the isolation of Campylobacter spp. from food, environmental samples and faeces. The use of a selective enrichment broth enhances the recovery of sub-lethally damaged organisms due to processing of foods, or if small numbers of campylobacters are present in heavily contaminated specimens. This broth has been shown to give appreciably better results than Preston Broth.

Method for reconstitution

Weigh 27.6 grams of powder, disperse in 1 litre of deionised w and allow to soak for 10 minutes. Swirl to mix and autoclave at 12 for 15 minutes. Cool to 47˚C, add 2 vials of selective supplem X131 ( X132 can be used as an alternative) reconstituted with 5m 50% alcohol and 50ml of saponin lysed horse blood, mix well dispense into sterile containers.

Appearance: Translucent, wine-red with a fine black suspension

pH: 7.4 ± 0.2

Minimum Q.C. organisms: Campylobacter jejuni

E. coli (inhibition) NCIMB 5003

Storage of Prepared Medium: Capped containers: 7 days at 2-in the dark.

Inoculation: Food homogenate is added to broth in a ratio of (w/v) in screw cap containers leaving a head space of 1.5 cm. faeces 1ml of a 10% suspension in Buffered Peptone Water LAB is added to 5ml of broth.

Incubation: Aerobically at 37˚C for 2-4 hours, followed by a fur 16-44 hours at 42˚C.

Subculture: Onto Campylobacter Blood Free Selective Med LAB 112.

References

Bolton, F.J. Personal Communication.

Hunt J.M., Abeyta C., and Tran T. (1998) Chapter 7 Campyloba in FDA Bacteriological Analytical Manual 8th Edition.