Vet-o-test

Feline-Parvovirus

VET509 

Only for export!

Principles
Vet-o-test Feline-Parvovirus is designed to detect the antigens of feline parvovirus in feline feces. Two monoclonal antibodies in the kit specifically bind with different epitopes of the antigens. After being absorbed into the cellulose pad, the antigens of feline parvovirus move and bind with gold-colloid complex of monoclonal anti-feline parvovirus of the conjugate pad, forming Ab-Ag complex. This complex, then, forms Ab-Ag-Ab direct sandwich binding with the antibody of another monoclonal anti-feline parvovirus in the nitrocellulose membrane. The test results can appear on Control and Test lines where the principles of immunochromatography are applied.

Characteristics

1. One-step rapid test of feline parvovirus antigens
2. Rapid test results between 5 ~ 10 minutes
3. Expensive equipment not required
4. Easy storage and maintenance
5. High-purity and high-quality materials of the test kit increases its sensitivity and specificity.

Materials (10 tests/kit)

1. Test 10 units
2. Diluent (buffer) 1㎖ x 10 units
3. Disposable dropper 10 units
4. Swabs for sample collection 10 units
5. Instruction for use 1 unit

Composition
Specimen well (S : for dropping), Test line (T), and Control line (C) are marked on the device. Inside it, the strip is composed of sample pad, conjugate pad, nitrocellulose membrane (test paper), and absorbent pad

Effect
Detection of feline parvovirus antigens from feline feces

Uses

1. Specimen
   Feline feces
2. Storage of specimen
   a) Store specimen at 2~8℃ for use within 24 hours or at -25℃ for later use.
   b) Keep the specimen at 22~25℃ before use.
3. Test procedure
   a) Collect feline feces with a swab and put the swab into a sample tube containing 1㎖ of buffer.
   Do not use sticky or a lump of feces.
   
   
   
   b) Swirl the buffer with the swab.
   
   
   
   c) Drop 100 ㎕ (4 drops) into the specimen well (S).
   
   
   
   
   
   d) Read the test results within 5~10 minutes.
   
   
   
   Consider the test results as invalid after 10 minutes
   
   
   
4. Interpretation of the results
   A purple band should appear on the control line regardless of the test result. The presence of another band on the test line determines the result.
   
   Control Line (C): The line should always appear regardless of the presence of the antigens of feline parvovirus. If this line does not appear, the test should be considered invalid. This is probably because of impure buffer and the lack of specimen. It should be tested again with new buffer.
   
   Test Line (T): The presence of antigens of feline parvovirus determines the test line.
   
   1) Negative: Control line only appears.
   
   
   
   2) Positive: Both Test and Control lines appear.
   
   
   
   3) Retesting
   a) Both Test and Control lines do not appear.
   
   
   
   b) Test line only appears.
   
   
   
5. Further examinations
   This test is for primary screening. Consult veterinarians for further necessary examinations to obtain clinical test results.
   

Precautions

1. Use for feline In-vitro diagnostic purposes only.
2. Use within 10 minutes after opening the pouch because the test kit is
3. very sensitive to moisture and its effect may diminish.
4. Be careful of not touching the result window.
5. Every specimen should be used with different droppers.
6. For testing, buffer included only should be used.
7. Deal with specimen carefully. They can deliver unknown virus or infectious bacteria.
8. Use disposable gloves when you suspect the infection cause by specimen. And wash your hands later.
9. Dispose solid wastes after sterilizing them at 121℃ for over 1 hour.
10. Do not use the kit when its pouch is torn, sealing is not good shape and expiration date passes.

Storage method
The test kits store at 2~30℃ can be used for 18 months after manufacturing. Do not keep them in refrigerator. However, if they are stored under cold circumstances, keep them at room temperature for 15 ~ 30 minutes before use.