Produkte/Mikrobiologie/Identifizierung/CARBA

CARBA

Diagnostic kit CARBA is intended for fast identification of bacteria producing carbapenemases

 

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By enzymatic hydrolysis of β-lactam ring, colouring of inoculated solution changes from negative red-pink to yellow, indicating positive test result of examined microorganism.

SUMMARY AND EXPLANATION
Diagnostic kit CARBA is intended for fast identification of bacteria producing carbapenemases.
 
PRINCIPLE
By enzymatichydrolysis of β-lactam ring, colouring of inoculated solution changes from negative red - pink to yellow, indicating positive test result of examined microorganism.
 
DIAGNOSTIC KIT CONTAINS
- CARBA „a“-substrate in 5 pc of microtubes eppendorf
- CARBA „b”-solution 5 ml
- information leaflet
 
RECCOMENDED PROCEDURE
1. Add 12 - 14 drops of solution CARBA „b“ to microtube CARBA „a“. Put one full 10 μl inoculation loop of bacteria
mass to prepared microtube and thoroughly homogenise. Use fresh 24 h bacterial culture from suitable cultivation medium (for example blood agar).
2. Evaluate result of test after 0,5 - 2 hours.
For the need of own proof function, use following recommended bacterial strains:
Bacterial strainCCM / ATCCColourInterpretation
Salmonella enterica
subsp. enterica
4419 / -yellowpositive
without bacteria-red - pinknegative
 
CCM: Czech Collection of Microorganisms, Masaryk University Brno, Kamenice 5,
625 00 Brno, Czech Republic, tel. +420549491430, e-mail: ccm@sci.muni.cz
 
WARNINGS AND SECURITY PRECAUTIONS
- For in vitro diagnostics use.
- For professional use only
- Follow the instructions exactly!
- All samples and inoculated products has to be considered aspotentially infectious and obvious safety valid regulations must berespected while handling.
- Before use, check if the packaging is intact. Do not use damagedkit.
- Leftovers of expired or devaluated reagent liquidate according to
regulations of your country. Packaging materials are recyclable.
 
STORAGE
Store CARBA at dark place at +2 to +8 °C. Expiration date is placed on the label of each package.

 

 



 
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